Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153607_g511 ACAGAGACCATTTGGACAGCCTTAGTGAAAGG(+AGGACA)GTACCTGGCGATGAAGGGGCACACAGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613153605_d85z GGAGGCACTGCTGTGTGCCCCTTCATCGCCAG(+AGGACA)GTACCCTTTCACTAAGGCTGTCCAAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613153604_5mlr TCTGAACTCCATTCTCAGCTGGGAAGGCTACG(+AGGACA)GATCCTTTCAGAGCGAGGTAAGGTTTTACAG NGG 10 8 17 8 24 0 100 5 10
    240613153603_q63j TCTGGCTGTAAAACCTTACCTCGCTCTGAAAG(+AGGACA)GATCCGTAGCCTTCCCAGCTGAGAATGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613153601_asc9 TTGAATGCATCCAACACATTTCCAGGAAGCTG(+AGGACA)GATCCTTCCTTCTAAGCAGACACTAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613153600_i6oq GAGGTTCTCCCTAGTGTCTGCTTAGAAGGAAG(+AGGACA)GATCCAGCTTCCTGGAAATGTGTTGGATGCA NGG 10 8 17 8 24 0 100 5 10
    240613153559_7k9g TCGATGTTTTGCAGGTTGCTCAAGCAGCAAAG(+AGGACA)GATCCTGCGGTGATGTGGCATTTTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613153557_ugp7 CCCACGGAGCAGAAAATGCCACATCACCGCAG(+AGGACA)GATCCTTTGCTGCTTGAGCAACCTGCAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613153554_bj1n GGATACCAGTAGTCCAGTCCAGTAGTGCCAGG(+AGGACA)GTACCAAACATGAAACAGCAGGGGTGATTTC NGG 10 8 17 8 24 0 100 5 10
    240613153553_hiti CTGTTGAAATCACCCCTGCTGTTTCATGTTTG(+AGGACA)GTACCCTGGCACTACTGGACTGGACTACTGG NGG 10 8 17 8 24 0 100 5 10