Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153718_xoqb AACATATAAGAGCTTTTGACAACTGATAACTG(+AGGACA)GATCCTTCCAAATAAATGCATTTTGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240613153716_ww2g TGTCTTATTTGCCAGCTGAGAAAATGTACATG(+AGGACA)GTACCATCATTAAGGTGGTCGGTATATAGGA NGG 10 8 17 8 24 0 100 5 10
    240613153715_6i77 TTTTTTCCTATATACCGACCACCTTAATGATG(+AGGACA)GTACCATGTACATTTTCTCAGCTGGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240613153714_qr43 CTCTGCTTTCTCGGGGTTGCCACAGCAGACTG(+AGGACA)GTACCTCATTTCACCACACCACTTAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240613153713_f12d ACACCTGTTCTTAAGTGGTGTGGTGAAATGAG(+AGGACA)GTACCAGTCTGCTGTGGCAACCCCGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613153711_0d95 ATTGCTTTAACCCAAGTTAAAACCAAGCTTGG(+AGGACA)GATCCCATGCTTGCTGAGATGATTGACGGCT NGG 10 8 17 8 24 0 100 5 10
    240613153709_edlz ATGACAGCCGTCAATCATCTCAGCAAGCATGG(+AGGACA)GATCCCAAGCTTGGTTTTAACTTGGGTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613153708_gkoi CCAGTGGAAAGCACTATCTAGATCACACTTTG(+AGGACA)GATCCCATTAGCCAAATGCACAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240613153706_ra9f TAATTTGGACATTCTGTGCATTTGGCTAATGG(+AGGACA)GATCCAAAGTGTGATCTAGATAGTGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613153704_ndja TGACTCAATTACAAATCGATTCCAATTCCAGG(+AGGACA)GTACCTCACAGCTCTATTGCTACCGCCAATT NGG 10 8 17 8 24 0 100 5 10