Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153445_rwid TCCTTCAGAATTTTCTTGGTTGTTGATTTGGG(+AGGACA)GATCCTCAATCTCTCAACTTAGTTCCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613153444_sxvx TTGACATTAGGGAACTAAGTTGAGAGATTGAG(+AGGACA)GATCCCCAAATCAACAACCAAGAAAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240613153442_1w1w TTGCTAGAGGAAAAATAGACAGAGAATCTAAG(+AGGACA)GTACCCCTGTCCCCAGAGCATGGTCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240613153441_lyy7 CCCGAGGAAATGACCATGCTCTGGGGACAGGG(+AGGACA)GTACCTTAGATTCTCTGTCTATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613153439_e8gi ACATCCACCATATGCCTGCTCCCCTGTCGCCG(+AGGACA)GTACCAAGTGAGATGTCTGTGTGGCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240613153437_yctx TGCAAAAACAAGCCACACAGACATCTCACTTG(+AGGACA)GTACCGGCGACAGGGGAGCAGGCATATGGTG NGG 10 8 17 8 24 0 100 5 10
    240613153436_h75j TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+AGGACA)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613153435_rqp4 TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+AGGACA)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613153434_ryql TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+AGGACA)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613153432_22fk TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+AGGACA)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10