Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153358_mxyt GTGGATACTGGTTTCAGAAATCTTGTGCCCAG(+AGGACA)GTACCCACAGTCCTCCTCACATACCTCATTT NGG 10 8 17 8 24 0 100 5 10
    240613153356_lc1j CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+AGGACA)GTACCTGGGCACAAGATTTCTGAAACCAGTA NGG 10 8 17 8 24 0 100 5 10
    240613153355_5yl0 AAGCTCGTGCTTGCAAGGCCAGGAAAGTCGTG(+AGGACA)GATCCCTGACAATGAAGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10
    240613153353_8vem AGCAACAATGAACCACTGCCTTCATTGTCAGG(+AGGACA)GATCCACGACTTTCCTGGCCTTGCAAGCACG NGG 10 8 17 8 24 0 100 5 10
    240613153351_m70i AATAGAGCCCTGAAGTGCTGACAGGGCTGAGG(+AGGACA)GATCCTTGGCTGCCATGTCCCACACAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613153349_0m2o GAAGAGTCTCTGTGTGGGACATGGCAGCCAAG(+AGGACA)GATCCCTCAGCCCTGTCAGCACTTCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613153348_2upn GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCTGCATATGGCAGTCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613153346_xqob GGATGGACCATCTAGTGACTGCCATATGCAGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613153343_76jf TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613153342_384z TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10