Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153310_woc8 ATAGGAAAGAACGGGAGATGATGGTGAGGCAG(+AGGACA)GATCCAACAGTCATCCTGCTGTGCTGAAATA NGG 10 8 17 8 24 0 100 5 10
    240613153308_hbgc ATTGTTATTTCAGCACAGCAGGATGACTGTTG(+AGGACA)GATCCTGCCTCACCATCATCTCCCGTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613153307_k6o5 ATCATCTGATGTGGGTGCTAGAAAACATCTGG(+AGGACA)GTACCCAACATGATCAGTACTATTGAGACAC NGG 10 8 17 8 24 0 100 5 10
    240613153305_iaif AGAATGTGTCTCAATAGTACTGATCATGTTGG(+AGGACA)GTACCCAGATGTTTTCTAGCACCCACATCAG NGG 10 8 17 8 24 0 100 5 10
    240613153304_ekd7 GGGTAAAATAAGTAAGTTTAAAGGGAATTAAG(+AGGACA)GATCCAGATAATACAGAAATGCCTCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613153303_u91q TCAGACCAAAGGAGGCATTTCTGTATTATCTG(+AGGACA)GATCCTTAATTCCCTTTAAACTTACTTATTT NGG 10 8 17 8 24 0 100 5 10
    240613153301_jdki ATCATCTGATGTGGGTGCTAGAAAACATCTGG(+AGGACA)GTACCCAACATGATCAGTACTATTGAGACAC NGG 10 8 17 8 24 0 100 5 10
    240613153300_bag5 AGAATGTGTCTCAATAGTACTGATCATGTTGG(+AGGACA)GTACCCAGATGTTTTCTAGCACCCACATCAG NGG 10 8 17 8 24 0 100 5 10
    240613153257_egb6 TCCCACACATGTGGAAGAGGCCGTGTCCCAGG(+AGGACA)GATCCACGTGCCTCTCTACCAATCACACCCG NGG 10 8 17 8 24 0 100 5 10
    240613153256_d467 ACGAGCGGGTGTGATTGGTAGAGAGGCACGTG(+AGGACA)GATCCCTGGGACACGGCCTCTTCCACATGTG NGG 10 8 17 8 24 0 100 5 10