Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153159_oliu CTCCAGCCACTCTGCTGTCTTCCAGGGTTGGG(+AGGACA)GATCCCGCAGGGTCAGCACCCCACACCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613153157_rvt9 GCTGGGAGAGGTGTGGGGTGCTGACCCTGCGG(+AGGACA)GATCCCCAACCCTGGAAGACAGCAGAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240613153155_spsa GCACCCAGCTGGCCTGATGTGGCTCTCCGGGG(+AGGACA)GTACCACAGAACACCCCTGTACATACCACAG NGG 10 8 17 8 24 0 100 5 10
    240613153153_5r3g CAGGGCTGTGGTATGTACAGGGGTGTTCTGTG(+AGGACA)GTACCCCCGGAGAGCCACATCAGGCCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613153152_f5a8 CAGGGTCTGCAAGTGGGCAAAGGCCTATTCAG(+AGGACA)GATCCTGGGTGGTGGAGGGGACAGCAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613153149_m01k AGTGACAACCTGCTGTCCCCTCCACCACCCAG(+AGGACA)GATCCTGAATAGGCCTTTGCCCACTTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240613153148_egn9 AGTGGGGAAGGTCTCAGGAGAGTCTACAGTAG(+AGGACA)GATCCTCTACTACTGCAGACTGGATTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613153147_hm72 CTAGAAAAGAAATCCAGTCTGCAGTAGTAGAG(+AGGACA)GATCCTACTGTAGACTCTCCTGAGACCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613153144_j0fj GCACACCTCCTCAGCTGAGGGGTCCAACCCTG(+AGGACA)GTACCGCTGGGATCCAGGGACCGAACCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613153142_ltpe ACCATGTGGGGTTCGGTCCCTGGATCCCAGCG(+AGGACA)GTACCAGGGTTGGACCCCTCAGCTGAGGAGG NGG 10 8 17 8 24 0 100 5 10