Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153111_bg2y GGCTCAGTTTCCCCGTGGGTGTCAGACAGTAG(+AGGACA)GTACCACCTACTCCAGGGCCTTCACTAGGGC NGG 10 8 17 8 24 0 100 5 10
    240613153110_irwa ATGAAGCCCTAGTGAAGGCCCTGGAGTAGGTG(+AGGACA)GTACCTACTGTCTGACACCCACGGGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240613153108_jt5d CCATCTGGGACTGTTATCCATCTGTGTCCATG(+AGGACA)GTACCAGGGAGCCCTGATCTCCCTGAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613153105_mkc3 CCGAGGAACTTCAGGGAGATCAGGGCTCCCTG(+AGGACA)GTACCATGGACACAGATGGATAACAGTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613153103_gxf8 AAGATGGCAGGCTGTCTAGGCACCTGGCCAGG(+AGGACA)GATCCAAAGGACATCAGGCTTGGGGTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240613153101_dign ACCCTAAAGGACCCCAAGCCTGATGTCCTTTG(+AGGACA)GATCCCTGGCCAGGTGCCTAGACAGCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613153059_0a8w GGTGTCTCACCTGCCCCACGCCAAGCCCTGAG(+AGGACA)GTACCAGGTGGACAGTAACGACCCCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613153058_dnn8 GGGTCAGAGCTGGGGTCGTTACTGTCCACCTG(+AGGACA)GTACCTCAGGGCTTGGCGTGGGGCAGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613153056_cmxa GCCTAGTTTGGCTCCTATGGAAGAGACAGCAG(+AGGACA)GATCCTTCCCGGTTGACTACTGTCACATTCA NGG 10 8 17 8 24 0 100 5 10
    240613153055_meuz ACACCTGAATGTGACAGTAGTCAACCGGGAAG(+AGGACA)GATCCTGCTGTCTCTTCCATAGGAGCCAAAC NGG 10 8 17 8 24 0 100 5 10