Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153036_ec7o TGAAGATACACCTAATCCTCAAAGAACAGGAG(+AGGACA)GATCCAGGGAGTGTGCAGGTTTGTTGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240613153034_92yo TCCTCCACCCCAACAAACCTGCACACTCCCTG(+AGGACA)GATCCTCCTGTTCTTTGAGGATTAGGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240613153033_pero CGGAGCAGTGTCAGAGGCCCAGGTGGATGCTG(+AGGACA)GTACCAGCAGATTAAACAGCAGCCATCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613153031_rdv9 TCCCAGCAGGATGGCTGCTGTTTAATCTGCTG(+AGGACA)GTACCAGCATCCACCTGGGCCTCTGACACTG NGG 10 8 17 8 24 0 100 5 10
    240613153030_99lx CAGAAATCAACCAAGGAGAGCACTTCGCAGAG(+AGGACA)GTACCAGGGATGGTGGCATCCTCTCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240613153028_m4ba CTGTCTGTGAAGAGAGGATGCCACCATCCCTG(+AGGACA)GTACCTCTGCGAAGTGCTCTCCTTGGTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613153027_2hv8 TGGTGTGGAGATGGCTTAGGCTAAAAGTTCTG(+AGGACA)GATCCCCGACTCCCCTAATGTTGATTGAATA NGG 10 8 17 8 24 0 100 5 10
    240613153025_c4ec TATGTTATTCAATCAACATTAGGGGAGTCGGG(+AGGACA)GATCCAGAACTTTTAGCCTAAGCCATCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613153023_2zgf ACAAGGACCCGCTTGTTTTGGTGCCATCTTGG(+AGGACA)GATCCCCATCTGCAGTACAAATACAGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613153021_fffw CTTCATAAAACTGTATTTGTACTGCAGATGGG(+AGGACA)GATCCCAAGATGGCACCAAAACAAGCGGGTC NGG 10 8 17 8 24 0 100 5 10