Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153141_hshr GGTAGGCTGTGCCTGCTAGACCTCCCCTGAAG(+AGGACA)GTACCTTGCTGTTGTCCCTGATGCAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613153140_j4tu TGCCATCAGCCTGCATCAGGGACAACAGCAAG(+AGGACA)GTACCTTCAGGGGAGGTCTAGCAGGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240613153139_4hms GAGGGCAGGATTGGGCATGGGGATGCTGTAAG(+AGGACA)GATCCAGCTTGTTTTGGCCACTTGGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240613153137_6u8a TGAGACCCATCCCAAGTGGCCAAAACAAGCTG(+AGGACA)GATCCTTACAGCATCCCCATGCCCAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240613153135_jcg7 TTAGCTTACTCACCCTTTGTCCCCTCCCCCAG(+AGGACA)GTACCATGTGCCGAGGCCTCAGACACAGCGT NGG 10 8 17 8 24 0 100 5 10
    240613153134_mcpm GGACTACGCTGTGTCTGAGGCCTCGGCACATG(+AGGACA)GTACCTGGGGGAGGGGACAAAGGGTGAGTAA NGG 10 8 17 8 24 0 100 5 10
    240613153132_28ar CGGCGCTGTCCAGTCTAAACCCCTGGAGGTTG(+AGGACA)GATCCTCAGCAGGGCTCCTGTTCTGGCGTCC NGG 10 8 17 8 24 0 100 5 10
    240613153131_n3cf TCATGGGACGCCAGAACAGGAGCCCTGCTGAG(+AGGACA)GATCCAACCTCCAGGGGTTTAGACTGGACAG NGG 10 8 17 8 24 0 100 5 10
    240613153129_9k16 CAGAGATGCCTGGCTGTCAGGACAGAAGGCCG(+AGGACA)GTACCCCAAGGTCCTGCTCTCAGGCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613153127_iuf4 CTTCTCTTCCAGCCTGAGAGCAGGACCTTGGG(+AGGACA)GTACCGGCCTTCTGTCCTGACAGCCAGGCAT NGG 10 8 17 8 24 0 100 5 10