Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613153502_n0by TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+AGGACA)GTACCAAATCCTAAGGAATGGCACTGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240613153501_4jqg ACAATTTGGGCAGTGCCATTCCTTAGGATTTG(+AGGACA)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613153458_rcl6 GTGGATACCGGTTTCAGAAATCTTGTGCCCAG(+AGGACA)GTACCCACAGTCCTCCTCACATACCTCATTT NGG 10 8 17 8 24 0 100 5 10
    240613153457_u0n6 CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+AGGACA)GTACCTGGGCACAAGATTTCTGAAACCGGTA NGG 10 8 17 8 24 0 100 5 10
    240613153455_20ou AAGCTCATGCTTGCAAGGCCAGGAAAGTCGTG(+AGGACA)GATCCCTGACAATGAGGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10
    240613153454_sgs4 AGCAACAATGAACCACTGCCCTCATTGTCAGG(+AGGACA)GATCCACGACTTTCCTGGCCTTGCAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240613153452_i9ps AATAGAGCCCTGAAGTGCTGACAGGGCTGAGG(+AGGACA)GATCCTTGGCTGCCATATCCCACAGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613153450_4rmk GAAGAGTCTCTCTGTGGGATATGGCAGCCAAG(+AGGACA)GATCCCTCAGCCCTGTCAGCACTTCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613153449_ktjn TAGATAGGCTCACAGGAGAACTTCATCTAGAG(+AGGACA)GATCCCTCTGTGAAGACTCTCCCTCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613153447_3ik0 GAAGTGACCTGGAGGGAGAGTCTTCACAGAGG(+AGGACA)GATCCTCTAGATGAAGTTCTCCTGTGAGCCT NGG 10 8 17 8 24 0 100 5 10