Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152917_7r9w TAGATCAGGAGGGCTGAATGGATTGGAAGGTG(+AGGACA)GATCCTATGTGGAGGATATGGGTGACTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613152916_rydg CTTGCTCCTAGTCACCCATATCCTCCACATAG(+AGGACA)GATCCACCTTCCAATCCATTCAGCCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613152914_6hkp TCGTGCTATATGGGTTATTTTGGTCTTGTAAG(+AGGACA)GATCCGTTCCTTTAAAATAATGGTCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613152913_a45z CTATATTCTAAGACCATTATTTTAAAGGAACG(+AGGACA)GATCCTTACAAGACCAAAATAACCCATATAG NGG 10 8 17 8 24 0 100 5 10
    240613152911_xigt TAGAAAACTTGCCTAGAATGTACAAGGCCTTG(+AGGACA)GATCCAAATCCCAGAACTGCAACTCTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613152910_do90 TCTTCTGGGGAGAGTTGCAGTTCTGGGATTTG(+AGGACA)GATCCAAGGCCTTGTACATTCTAGGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613152909_d2ez GACATTGCGAGCGTCAGTGGCCGCTGGTAGCG(+AGGACA)GTACCTTCTGGACACGCACCTGCTATCTTGC NGG 10 8 17 8 24 0 100 5 10
    240613152908_66ws CCTTTGCAAGATAGCAGGTGCGTGTCCAGAAG(+AGGACA)GTACCGCTACCAGCGGCCACTGACGCTCGCA NGG 10 8 17 8 24 0 100 5 10
    240613152907_thvx ATATTACATATGCACTGATTTTTTTTTTCATG(+AGGACA)GTACCTAAAATGAAGGAGAATTTTGACATCT NGG 10 8 17 8 24 0 100 5 10
    240613152905_a8wz ATCCCAGATGTCAAAATTCTCCTTCATTTTAG(+AGGACA)GTACCATGAAAAAAAAAATCAGTGCATATGT NGG 10 8 17 8 24 0 100 5 10