Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152845_xoep TTCAAAACAATGAAATAAAACCTAAGCCGTAG(+AGGACA)GTACCTAAAATGAGGTTATTTCTCAACCCCT NGG 10 8 17 8 24 0 100 5 10
    240613152844_mmqu GGATAAGGGGTTGAGAAATAACCTCATTTTAG(+AGGACA)GTACCTACGGCTTAGGTTTTATTTCATTGTT NGG 10 8 17 8 24 0 100 5 10
    240613152843_ueau CAAGAGTCAGTGAAAGAGTCCCGAGAGCGCTG(+AGGACA)GATCCCCATCCTTGTCCAGGGTGAAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613152841_dbxd TCCCAAGTGACTTCACCCTGGACAAGGATGGG(+AGGACA)GATCCAGCGCTCTCGGGACTCTTTCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240613152839_1o31 TCTGTCCTTAGACACACCAGAAAAGGGCATCG(+AGGACA)GATCCCATTACAGATAGCTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613152838_99zz ACCACATGGTGGCTCACAGCTATCTGTAATGG(+AGGACA)GATCCGATGCCCTTTTCTGGTGTGTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240613152836_xp6s CAGCCTCGAGAAATTTCTCCTATTATATTTGG(+AGGACA)GATCCAGCATGTCCCAAGACAGGGACGACTC NGG 10 8 17 8 24 0 100 5 10
    240613152835_m8b8 TTATTGAGTCGTCCCTGTCTTGGGACATGCTG(+AGGACA)GATCCCAAATATAATAGGAGAAATTTCTCGA NGG 10 8 17 8 24 0 100 5 10
    240613152834_lv5q TTCTGGGGGCAGAGTTCTGGTTACTTTTCACG(+AGGACA)GATCCGCAGAAGAACTGCCTAGGTGCCTAGG NGG 10 8 17 8 24 0 100 5 10
    240613152833_f5mo ACACCCCTAGGCACCTAGGCAGTTCTTCTGCG(+AGGACA)GATCCGTGAAAAGTAACCAGAACTCTGCCCC NGG 10 8 17 8 24 0 100 5 10