Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152904_ud1z CAAAGTGCCTTTGTCTGCCTGGAGTCAACTTG(+AGGACA)GATCCATTGGAAACATAGAAAGGGTCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613152902_hsct CTGTCCCCTGGACCCTTTCTATGTTTCCAATG(+AGGACA)GATCCAAGTTGACTCCAGGCAGACAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613152900_1t6m CAGGTGGCTGTGGGGGACAGAAGAGGGTGCCG(+AGGACA)GATCCCCTGGAGCTGGAGTTAGGCTTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613152858_sdg0 TTAGTAACTAAAGCCTAACTCCAGCTCCAGGG(+AGGACA)GATCCGGCACCCTCTTCTGTCCCCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240613152857_7gf1 AGCAGAGCCCTGGCCCTATTAGCAGCACAGTG(+AGGACA)GTACCCGAGGGTCTCTGTCAACAAGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240613152854_s302 TCATTTCCTGTCTTGTTGACAGAGACCCTCGG(+AGGACA)GTACCACTGTGCTGCTAATAGGGCCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613152853_idst CACACACAGCACCCATGCTAGCACGCACAGAG(+AGGACA)GATCCCGTTGATCACTCCAGAAGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240613152851_aimb ACCCAGGTCACCTCTTCTGGAGTGATCAACGG(+AGGACA)GATCCTCTGTGCGTGCTAGCATGGGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613152849_9g8b AATTAAATGTTGTCCTTATAAGAGTTGCCTTG(+AGGACA)GTACCAGGAGAGCCAGGGCTATACAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613152847_smxr CAGGGTTTCTCTGTATAGCCCTGGCTCTCCTG(+AGGACA)GTACCAAGGCAACTCTTATAAGGACAACATT NGG 10 8 17 8 24 0 100 5 10