Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150650_vlh9 CATTCATAACCGGAAGTAGACGAGGAGTACAG(+AGGACA)GTACCGACTGGCAACGTCCACAGGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613150648_l6tb TATTTCTTTGCACTCTAACCTGCAAGGCATGG(+AGGACA)GTACCCTTGGCAGACCCCTGGTCGGAACTAG NGG 10 8 17 8 24 0 100 5 10
    240613150646_un3z AGGGGCTAGTTCCGACCAGGGGTCTGCCAAGG(+AGGACA)GTACCCATGCCTTGCAGGTTAGAGTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240613150644_k1wk AACTGGGTTTGCTGAATATACCTGAAGGCCTG(+AGGACA)GTACCTGGGAGGTAGAGGTGGAGGCGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613150641_6nww CTGATCTTCCCGCCTCCACCTCTACCTCCCAG(+AGGACA)GTACCAGGCCTTCAGGTATATTCAGCAAACC NGG 10 8 17 8 24 0 100 5 10
    240613150639_k01o ACCACATAGTGGCTCACAACCATCTGCAATGG(+AGGACA)GATCCGATGCTCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613150638_r4x8 GCTGTCTTCAGACACACCAGAAGAGAGCATCG(+AGGACA)GATCCCATTGCAGATGGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240613150637_bu83 GCTTGTTTTCTCTTTGTAAAGGACAAACTATG(+AGGACA)GTACCAGAATCTGCCAAAGATCCCCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613150636_q570 TGAGATGAGAAGGGGATCTTTGGCAGATTCTG(+AGGACA)GTACCATAGTTTGTCCTTTACAAAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613150634_00m8 CTTGCTTAGAGCCAGAAGGGATGATGTCCTGG(+AGGACA)GATCCAAGACCAGAGGGACAGCATCACTGAG NGG 10 8 17 8 24 0 100 5 10