Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150559_xskk ATATTCTAGAGAGCACAGCAACTGCTCTGGTG(+AGGACA)GATCCTTTGGGGGTCAAGGGTCCCGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613150556_s56l GCTCCATTATTCCCCACACAAGACTCCCCTGG(+AGGACA)GTACCACGGAGAACTCCTCTAAAACCTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613150554_fumv GCACCTAAGAGGTTTTAGAGGAGTTCTCCGTG(+AGGACA)GTACCCAGGGGAGTCTTGTGTGGGGAATAAT NGG 10 8 17 8 24 0 100 5 10
    240613150552_gmyx GCCGCCTGGAAGCGCTCAGTGCCACGGCTGGG(+AGGACA)GATCCTAGATCCTGCTGCATGGACCCTATAA NGG 10 8 17 8 24 0 100 5 10
    240613150551_5t45 ACAATTTATAGGGTCCATGCAGCAGGATCTAG(+AGGACA)GATCCCCAGCCGTGGCACTGAGCGCTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613150548_60ls TCAATAATTCTGTGCCTCGCATTCCTTCCTGG(+AGGACA)GATCCCAGCCCTGACTTTCCAGAAGAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613150547_j1hb AACACTTGACTCTTCTGGAAAGTCAGGGCTGG(+AGGACA)GATCCCAGGAAGGAATGCGAGGCACAGAATT NGG 10 8 17 8 24 0 100 5 10
    240613150545_kj5a AGGGACGGGAGCAAAGAGGCTGCCGTAACTAG(+AGGACA)GTACCTAGTGGAGGGAAATGGTCACCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613150544_ugid CATCAGGCTTGGTGACCATTTCCCTCCACTAG(+AGGACA)GTACCTAGTTACGGCAGCCTCTTTGCTCCCG NGG 10 8 17 8 24 0 100 5 10
    240613150543_yqdn AAAGCTGTCACATGAAAGCCATCCCAGAAATG(+AGGACA)GTACCTTTTTTTTTTTTTTTTTCCTAAAGAC NGG 10 8 17 8 24 0 100 5 10