Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150451_k9ck AAAGAGAAGGTCTGAGAATCTGCCTTCCGCTG(+AGGACA)GTACCATTTCCCTCTCCCTTTCGGGGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613150450_vd0o CTTCTTCCTTCTTTTTGTGAACAAATCAATAG(+AGGACA)GATCCTTTTTACTGTCAGACTTTCCCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613150449_ka7o ACAAATCAAGGGGAAAGTCTGACAGTAAAAAG(+AGGACA)GATCCTATTGATTTGTTCACAAAAAGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613150447_i32l TCACAGGTCTTTGTGAGGACTCCAGATCTTGG(+AGGACA)GATCCACCACTTAGCATTATAATAGATAGCA NGG 10 8 17 8 24 0 100 5 10
    240613150445_s4h6 GGTCTTGCTATCTATTATAATGCTAAGTGGTG(+AGGACA)GATCCCAAGATCTGGAGTCCTCACAAAGACC NGG 10 8 17 8 24 0 100 5 10
    240613150444_1h4o ACTTCAAAAGTTGGTTTTTCTCCTTCTACTGG(+AGGACA)GATCCAAGGATACAACTCAGGTTATCAGACT NGG 10 8 17 8 24 0 100 5 10
    240613150442_i9eh GAACTAGTCTGATAACCTGAGTTGTATCCTTG(+AGGACA)GATCCCAGTAGAAGGAGAAAAACCAACTTTT NGG 10 8 17 8 24 0 100 5 10
    240613150440_jknd ATTGGAAGAGCAACATGGGCCACCCCTGCTGG(+AGGACA)GTACCCAACTTATGAGCAAGAGAATGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613150439_hixv TGGAAGCTTCCATTCTCTTGCTCATAAGTTGG(+AGGACA)GTACCCAGCAGGGGTGGCCCATGTTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613150437_z1q0 GCTCTGCGCACAGTTTGTTCACTAGAGGCCTG(+AGGACA)GTACCTGTCTTCTCTCAGGCCTCTGCACTCA NGG 10 8 17 8 24 0 100 5 10