Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150436_5739 CCGTGTGAGTGCAGAGGCCTGAGAGAAGACAG(+AGGACA)GTACCAGGCCTCTAGTGAACAAACTGTGCGC NGG 10 8 17 8 24 0 100 5 10
    240613150433_7ba2 TGGATCAGGAGCCATATTAAGATGACTTTGGG(+AGGACA)GATCCCAGCAGTCCCTACGTGATCACATAGC NGG 10 8 17 8 24 0 100 5 10
    240613150432_rti4 CAGGAGCTATGTGATCACGTAGGGACTGCTGG(+AGGACA)GATCCCCAAAGTCATCTTAATATGGCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613150430_27zx ATGATTACTAGCACTAGAAGCCAACAGCAAAG(+AGGACA)GATCCCGCGCGCAGTTGCTCTTGTTTAATCC NGG 10 8 17 8 24 0 100 5 10
    240613150429_qgc9 AACCTGGATTAAACAAGAGCAACTGCGCGCGG(+AGGACA)GATCCTTTGCTGTTGGCTTCTAGTGCTAGTA NGG 10 8 17 8 24 0 100 5 10
    240613150427_asz1 TGGTCCATTTAAAGTTTCCCAGCTATGGGGTG(+AGGACA)GTACCAGTTCTGGACAAGTGCCACTGCATCA NGG 10 8 17 8 24 0 100 5 10
    240613150426_ej84 TACTATGATGCAGTGGCACTTGTCCAGAACTG(+AGGACA)GTACCACCCCATAGCTGGGAAACTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240613150424_69ue GGGCGGGAGTAAGGAAATGCAGGAGTTGACCG(+AGGACA)GATCCTTGTTCCTCACCTTGTACACTCACTT NGG 10 8 17 8 24 0 100 5 10
    240613150423_r7j5 AGTTTAAGTGAGTGTACAAGGTGAGGAACAAG(+AGGACA)GATCCGGTCAACTCCTGCATTTCCTTACTCC NGG 10 8 17 8 24 0 100 5 10
    240613150422_jo7u CATGCTATATGTATACTTCTGTTTTCTGTTGG(+AGGACA)GTACCTTGCAATGCATTCTATGCCTCATTAA NGG 10 8 17 8 24 0 100 5 10