Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150526_b93p ACAGGGATGTCGAGATACAATCCCGACAGAGG(+AGGACA)GTACCTTCTGCAGCTCAATTCTTACAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613150525_l50l AGTCATTTTGAATTTGGTCTGTGCCTTCTCTG(+AGGACA)GATCCCTCCCTAGCCTGTGCCCCGAGCCGTC NGG 10 8 17 8 24 0 100 5 10
    240613150523_5p0z CTATGGACGGCTCGGGGCACAGGCTAGGGAGG(+AGGACA)GATCCAGAGAAGGCACAGACCAAATTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613150521_zw4z CACCCACTTGAGGTCTCCCGACATTCACCCTG(+AGGACA)GTACCACACCACTTGATCTCCAGAGCAATCT NGG 10 8 17 8 24 0 100 5 10
    240613150520_32n3 GGGCCAGATTGCTCTGGAGATCAAGTGGTGTG(+AGGACA)GTACCAGGGTGAATGTCGGGAGACCTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240613150518_590d TCGCCAGTTCTGTTTGGAGTCCGTGTAGGTGG(+AGGACA)GATCCTGCTGTGTGTAGCACTGGCCTGGACA NGG 10 8 17 8 24 0 100 5 10
    240613150516_v9za GGAGATGTCCAGGCCAGTGCTACACACAGCAG(+AGGACA)GATCCCACCTACACGGACTCCAAACAGAACT NGG 10 8 17 8 24 0 100 5 10
    240613150515_udox TGTAGGGGGTTGAGAGGAAGGGAATGAGCCAG(+AGGACA)GTACCCTGCATTCCAAGGGAGTTTCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613150513_l1m6 CTTTGCACACTGAAACTCCCTTGGAATGCAGG(+AGGACA)GTACCTGGCTCATTCCCTTCCTCTCAACCCC NGG 10 8 17 8 24 0 100 5 10
    240613150511_l3cj GAAGGTTTAGAGAATCCGATGGGCGCTGCCTG(+AGGACA)GATCCCGGCATAGCCCCAGACACTTGGTTCC NGG 10 8 17 8 24 0 100 5 10