Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613150632_4ljl CTCCCCTCAGTGATGCTGTCCCTCTGGTCTTG(+AGGACA)GATCCCAGGACATCATCCCTTCTGGCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240613150631_uacq GTACATGAGTGTGGAAGTCTGAGCTATATTTG(+AGGACA)GTACCCGATAGGATACTTTAGGTATCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240613150630_c4gs ACTGAATCCTGATACCTAAAGTATCCTATCGG(+AGGACA)GTACCAAATATAGCTCAGACTTCCACACTCA NGG 10 8 17 8 24 0 100 5 10
    240613150628_sc7k CCCCAGGAAGGCAGTACCCTGCCCCTGGGAGG(+AGGACA)GTACCACTCACATGGAACCAGAGGAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613150626_fx0p ATGGAGCAGCTTCCTCTGGTTCCATGTGAGTG(+AGGACA)GTACCCTCCCAGGGGCAGGGTACTGCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613150624_x5g3 CCACTCAAGCCAACCGTACAAGCTCCCTGGGG(+AGGACA)GATCCCACTGGGGAAACCAATGCTATAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613150623_epws CTCTGAAGCTATAGCATTGGTTTCCCCAGTGG(+AGGACA)GATCCCCCAGGGAGCTTGTACGGTTGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613150621_a7w3 ATGGAACAGAGATCGGTATCCTATTGGGAAAG(+AGGACA)GTACCCGGTCTAACCCTTCCAATCTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240613150619_qy3y CATTTTAGGATAGATTGGAAGGGTTAGACCGG(+AGGACA)GTACCTTTCCCAATAGGATACCGATCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613150617_6bhd GTCCCCCACATCCCCTTCTGTACCCCTTCCTG(+AGGACA)GATCCCCACTGCTGGGGCCAGGAAAGCCCTC NGG 10 8 17 8 24 0 100 5 10