Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141706_b7ah TAGGGGGCAATGGCCTAGTTGTCCTAGAGGAG(+AGGACA)GATCCCATGGCTGGCCAGATCAGTAGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613141704_zgyd CAGTATCCACGGTGGGTACTGAGGAAGGGGTG(+AGGACA)GTACCCACCTCAGATGGGAGTTGAGACCATT NGG 10 8 17 8 24 0 100 5 10
    240613141702_g3c6 GTTGCAATGGTCTCAACTCCCATCTGAGGTGG(+AGGACA)GTACCACCCCTTCCTCAGTACCCACCGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613141700_kezk CAAAAGTTGACATCAACCAGGTGCAGTACTGG(+AGGACA)GTACCAGTAGCCTGAGCTGAAGCAGGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613141659_2wtg TCTCAAGCCTCCTGCTTCAGCTCAGGCTACTG(+AGGACA)GTACCCAGTACTGCACCTGGTTGATGTCAAC NGG 10 8 17 8 24 0 100 5 10
    240613141658_83g1 CAATAGGACTTGCAGGAATCCCTCCGATGGTG(+AGGACA)GTACCCCGTGATTGTCGGCCAGGCTCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613141656_lbj4 GTGAGAACTAGAGCCTGGCCGACAATCACGGG(+AGGACA)GTACCACCATCGGAGGGATTCCTGCAAGTCC NGG 10 8 17 8 24 0 100 5 10
    240613141654_q1zo ATTGAAGCATCGGACTTTATAGGTGCTCCCTG(+AGGACA)GTACCAACTCAGCCAGGTAGTCTATCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240613141652_8pch GCTGTGCCTAGATAGACTACCTGGCTGAGTTG(+AGGACA)GTACCAGGGAGCACCTATAAAGTCCGATGCT NGG 10 8 17 8 24 0 100 5 10
    240613141651_la4z AAAGCAGTCAGCCAGCACTTCCTGTGCTCATG(+AGGACA)GATCCAGAAGACTCTGTTCTCACGACCATTT NGG 10 8 17 8 24 0 100 5 10