Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141601_hblp TGAGAATGTGGGAAGCTTGTTGTTCAGAGGAG(+AGGACA)GTACCCTGTTGAGAAAATCTTCAAATGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613141600_sa31 TGAAATGGCCTCAGCAAGAATAAAAGATGAAG(+AGGACA)GATCCTGTGGACTTTCTTCTCCTGCTACAAA NGG 10 8 17 8 24 0 100 5 10
    240613141558_uo7x ATACATTTGTAGCAGGAGAAGAAAGTCCACAG(+AGGACA)GATCCTTCATCTTTTATTCTTGCTGAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613141557_j85r GCTGTCATCAGACACATCAGAAGAGGGCATCG(+AGGACA)GATCCCATTACAGATGCTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613141555_fb0o ACCACATGGTGGCTCACAAGCATCTGTAATGG(+AGGACA)GATCCGATGCCCTCTTCTGATGTGTCTGATG NGG 10 8 17 8 24 0 100 5 10
    240613141553_thj2 CCATTATGACCCCGTTTCCACACATCCGCCTG(+AGGACA)GTACCGTGCTCACCATGAACTATGCAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613141552_1ieu GCTTTCTTTCTGCATAGTTCATGGTGAGCACG(+AGGACA)GTACCAGGCGGATGTGTGGAAACGGGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240613141550_2zse CCAGTTGGCTGGGACTACACTCATTTATTCGG(+AGGACA)GATCCATATACACTCACAAGCATTTACAGCA NGG 10 8 17 8 24 0 100 5 10
    240613141549_962k CCCCTTGCTGTAAATGCTTGTGAGTGTATATG(+AGGACA)GATCCCGAATAAATGAGTGTAGTCCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613141547_ucpp CCATTATAACCCTGTTTCCACACGTCTGCCTG(+AGGACA)GTACCATGCTCACCATTAACAAATTATGCCG NGG 10 8 17 8 24 0 100 5 10