Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141454_pjkg CCTAGAAGGGTTGAGTTTTCTGTAGGCTCCAG(+AGGACA)GATCCACCTTTTCTTCTATAGACACTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613141452_imb9 GGGAGCCAGTGGTTTCTGAAGACTGTCCAGGG(+AGGACA)GATCCCTAGTTCAGAGTGGCCCCATCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613141450_very CATGTTGCCTGATGGGGCCACTCTGAACTAGG(+AGGACA)GATCCCCTGGACAGTCTTCAGAAACCACTGG NGG 10 8 17 8 24 0 100 5 10
    240613141448_t9xr AGGGAGGTGTGCGATGTTAGCCTTCTGCTTGG(+AGGACA)GATCCTTTGACCGGGAGGCCTGTAGTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613141447_4exh ACTTCAGGACACTACAGGCCTCCCGGTCAAAG(+AGGACA)GATCCCAAGCAGAAGGCTAACATCGCACACC NGG 10 8 17 8 24 0 100 5 10
    240613141445_dgeh GGCCCTGCTAGATGCTGACATCAGCCCCAGGG(+AGGACA)GTACCTTTCTGCTCTGCAGGACTGATCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613141444_gfi7 GGAGGATGAGATCAGTCCTGCAGAGCAGAAAG(+AGGACA)GTACCCCTGGGGCTGATGTCAGCATCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240613141442_xal1 CTTTCCTGGAACTCAAGATCATCTTGCCTTAG(+AGGACA)GATCCCAAACACTGCAATTACAGACTTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613141440_zvmi GAGTGGCACAAGTCTGTAATTGCAGTGTTTGG(+AGGACA)GATCCTAAGGCAAGATGATCTTGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613141438_hiol GGATCCCTCCTGGGCTTGTCAGAGAGTTTTGG(+AGGACA)GTACCAAAGAGGTCTAGTAGACTCCCCAGAA NGG 10 8 17 8 24 0 100 5 10