Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141405_ppfa CTAGGCAGGACAGACAAAGGCGGGACTTTTAG(+AGGACA)GATCCCAGGGCTTGGAAGGGAGGGTAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240613141404_q2yi GATTCTCTGCTCTTTGTATGACCGGGTGAAAG(+AGGACA)GTACCTGTCGTCTTTACAAGTTAGTTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240613141402_n9r4 ATACAGTCCAAACTAACTTGTAAAGACGACAG(+AGGACA)GTACCTTTCACCCGGTCATACAAAGAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240613141401_i9xg TATAAGTTTAGTATACAATGGATCAAATTAAG(+AGGACA)GATCCTGCTCTCTTTCCTTTGGAGGTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613141400_x22z ACGTCGAACTACCTCCAAAGGAAAGAGAGCAG(+AGGACA)GATCCTTAATTTGATCCATTGTATACTAAAC NGG 10 8 17 8 24 0 100 5 10
    240613141358_c45l CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AGGACA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613141357_9rxc GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AGGACA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613141355_gnrz CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AGGACA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613141353_t1r3 GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AGGACA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613141352_hzgh CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AGGACA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10