Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141302_kos3 AATGGTTGGGGACCTATACTCGGAATATTACG(+AGGACA)GTACCACTTGTTTTCCCACGGTATTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613141301_klud GGAAGGGCTGGGGGAGGGGGAGTCAGTGCGAG(+AGGACA)GTACCTCGAAGGCTATTGCTTTCTAGGGATT NGG 10 8 17 8 24 0 100 5 10
    240613141259_i392 GTGAAAATCCCTAGAAAGCAATAGCCTTCGAG(+AGGACA)GTACCTCGCACTGACTCCCCCTCCCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613141258_nxye TCTAGAAGATCCCTGTGCCCCCAGGGGAGGCG(+AGGACA)GATCCTAAGGGGTGAGGGATAAGGATTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613141256_pgw4 CCATGCCCCAATCCTTATCCCTCACCCCTTAG(+AGGACA)GATCCGCCTCCCCTGGGGGCACAGGGATCTT NGG 10 8 17 8 24 0 100 5 10
    240613141254_d7ij AAAAAACTCAGGGTGAGAGGAACGAGGAAGGG(+AGGACA)GATCCAATTTAGCTCAGCATCCTAAAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613141253_tzda AAGAAAACACTTTAGGATGCTGAGCTAAATTG(+AGGACA)GATCCCCTTCCTCGTTCCTCTCACCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240613141252_hvj3 CTTTTCAGATAGCCCTGGCTGGTGGGGGAATG(+AGGACA)GATCCCTGAGAACACGGGCATGTCACACAGG NGG 10 8 17 8 24 0 100 5 10
    240613141250_4rgk CATTCCCTGTGTGACATGCCCGTGTTCTCAGG(+AGGACA)GATCCATTCCCCCACCAGCCAGGGCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240613141248_h9tc GCAGGGGGAGAGAGGAGCTGAGGAAATGTGGG(+AGGACA)GATCCTGGGGGCTCGGACCGGGGTGAGTGTC NGG 10 8 17 8 24 0 100 5 10