Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141245_w8u3 ACATAGACACTCACCCCGGTCCGAGCCCCCAG(+AGGACA)GATCCCCACATTTCCTCAGCTCCTCTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613141244_nrhu CTATAGATGGAGATCTGAAGATTAGACTCTAG(+AGGACA)GATCCACAGATGAGAGAGTATACGTGACATT NGG 10 8 17 8 24 0 100 5 10
    240613141243_a1d7 AGGCAAATGTCACGTATACTCTCTCATCTGTG(+AGGACA)GATCCTAGAGTCTAATCTTCAGATCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240613141242_8qg1 CCTCACCAGGTAGCTCTCTAGCCCCAAGAAAG(+AGGACA)GATCCCACCAGAAACAGTATCAGATGTCATA NGG 10 8 17 8 24 0 100 5 10
    240613141240_w86x TAATTTATGACATCTGATACTGTTTCTGGTGG(+AGGACA)GATCCTTTCTTGGGGCTAGAGAGCTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240613141238_dw16 CAAGCTGCTGAAACCTTTAAAATGTATCAAGG(+AGGACA)GATCCATAGCCTCTACCATCCGGAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240613141237_ctoi ATCCTTTAGCATTCCGGATGGTAGAGGCTATG(+AGGACA)GATCCCTTGATACATTTTAAAGGTTTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613141236_k7h8 TAATAGTTGCAATGTAATTTATGGAGCACTTG(+AGGACA)GTACCACATTGTGTCTATTCTTTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613141234_z6vc CACAAGCAGCTTGAAAGAATAGACACAATGTG(+AGGACA)GTACCAAGTGCTCCATAAATTACATTGCAAC NGG 10 8 17 8 24 0 100 5 10
    240613141232_xwxz ACATTGTCTGTGTACATTCTTTCAAACTCCTG(+AGGACA)GTACCCTCCACACACTGCTCTGCTTACTATT NGG 10 8 17 8 24 0 100 5 10