Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141230_yxc7 GCAACAATAGTAAGCAGAGCAGTGTGTGGAGG(+AGGACA)GTACCAGGAGTTTGAAAGAATGTACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240613141229_dqo4 ATCAGCATGCCTGCCTCATGGGATTAAAGGTG(+AGGACA)GTACCACCATGCCTGGATCTAATTAAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613141227_r6kn TCTACCCAGCTTAATTAGATCCAGGCATGGTG(+AGGACA)GTACCACCTTTAATCCCATGAGGCAGGCATG NGG 10 8 17 8 24 0 100 5 10
    240613141226_hwk8 GTCAGGCCATTCACAGCAATATACCACTCCTG(+AGGACA)GTACCAACTTCTGTCTTAGTTAGGGTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240613141224_oqsx CAGCAGTAAAACCCTAACTAAGACAGAAGTTG(+AGGACA)GTACCAGGAGTGGTATATTGCTGTGAATGGC NGG 10 8 17 8 24 0 100 5 10
    240613141223_13a7 CATTAATTACTAAAGCTCTTTATATAGGAGAG(+AGGACA)GATCCTCTTGAGATTTGTCCATCAATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613141222_uvl7 ACATGCCAGCATTGATGGACAAATCTCAAGAG(+AGGACA)GATCCTCTCCTATATAAAGAGCTTTAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240613141220_a65m ATTATGTAAACTCTAGACCCCGAAACTTCCAG(+AGGACA)GATCCTCTGTTCTCAGATGCTCTGGTGATCT NGG 10 8 17 8 24 0 100 5 10
    240613141219_hz0n TAGGCAGATCACCAGAGCATCTGAGAACAGAG(+AGGACA)GATCCTGGAAGTTTCGGGGTCTAGAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240613141217_e56z TCACTCAGGATATTGTTACAGGACATGCAGGG(+AGGACA)GATCCAATCCCTTCCACACCTTCCGACCCTT NGG 10 8 17 8 24 0 100 5 10