Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141125_4jvs TCAGCTCCATCTGCAGACAAGCTCTAGCAAAG(+AGGACA)GATCCTTGAAGACTTTTGACACATCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240613141124_zjg5 GACAGTCATCTGATGTGTCAAAAGTCTTCAAG(+AGGACA)GATCCTTTGCCAGAGCTTGTCTGCAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240613141122_9juy TCAGGTCCATCTGCAGACAAGCTCTGGCAAAG(+AGGACA)GATCCTTGAAGACTTTTGACACATCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240613141121_9l97 GCCAGTCATCTGATATGTCAAAAGTATTCAAG(+AGGACA)GATCCTTTTCCAGAGCTTGTCTGCAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240613141120_de2d TCAGGTCCATCTGCAGACAAGCTCTGGAAAAG(+AGGACA)GATCCTTGAATACTTTTGACATATCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240613141118_9cfn AACTGAGTAGCAGGAGGAAAAGGAGGGCCAGG(+AGGACA)GATCCTGGTGTAGTGAGCAGGGTGGTTAGCT NGG 10 8 17 8 24 0 100 5 10
    240613141116_z4wp TGTTAAGCTAACCACCCTGCTCACTACACCAG(+AGGACA)GATCCCTGGCCCTCCTTTTCCTCCTGCTACT NGG 10 8 17 8 24 0 100 5 10
    240613141114_5tfa TAGCCTTCACCCAGAAGCATGCATGGCCTGAG(+AGGACA)GTACCCATGGGTTATCTAACTTCTCAGAATC NGG 10 8 17 8 24 0 100 5 10
    240613141112_7zco GAGTGGATTCTGAGAAGTTAGATAACCCATGG(+AGGACA)GTACCTCAGGCCATGCATGCTTCTGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240613141110_z5zv GCAATCTTCTTGCAATCCCTGACCTATTTGGG(+AGGACA)GTACCTTAGAACCTCCTCTTGGCTAGAGAGG NGG 10 8 17 8 24 0 100 5 10