Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141035_g4p3 ATTAGGCCAATGCAATGACTCAGCAGGTAAAG(+AGGACA)GTACCTCTTGCCAATCCTGATGACCCGAATT NGG 10 8 17 8 24 0 100 5 10
    240613141033_cxl9 GATGGTCTTCGACTGTAAAATGGGGTCTGTGG(+AGGACA)GTACCATCATTCTCTGGGCCGGTTGTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613141032_vgkj ACACCTCAGGACAACCGGCCCAGAGAATGATG(+AGGACA)GTACCCACAGACCCCATTTTACAGTCGAAGA NGG 10 8 17 8 24 0 100 5 10
    240613141031_54je AAAAGGTATCTGGGTAGCTCAAGCCAAGCAGG(+AGGACA)GATCCTGGTTATTTCTGTTGGCTCTTTAACA NGG 10 8 17 8 24 0 100 5 10
    240613141029_286i AATGATGTTAAAGAGCCAACAGAAATAACCAG(+AGGACA)GATCCCTGCTTGGCTTGAGCTACCCAGATAC NGG 10 8 17 8 24 0 100 5 10
    240613141027_t0rg TTCAATCTCAGTCTTTCTAGGGGAAAAAACTG(+AGGACA)GATCCAGAATTACTCAGACACCTTTCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613141026_0v9z CTGAGAAAGAGAAAGGTGTCTGAGTAATTCTG(+AGGACA)GATCCAGTTTTTTCCCCTAGAAAGACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613141025_9hwx GTAAGGAAGCAGAGCCCAGGAGCTCAAAGCTG(+AGGACA)GTACCCAGAAGAGCCAGCAGGGCCAGGCTAA NGG 10 8 17 8 24 0 100 5 10
    240613141023_pjyt AGTCCTTAGCCTGGCCCTGCTGGCTCTTCTGG(+AGGACA)GTACCAGCTTTGAGCTCCTGGGCTCTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613141021_t159 GGTGACCTGCCATCTGTGACAAGCTTTCTCGG(+AGGACA)GTACCTCTCCCCACCTCATTCCTTTAGGCTA NGG 10 8 17 8 24 0 100 5 10