Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141020_axfu CTCCATAGCCTAAAGGAATGAGGTGGGGAGAG(+AGGACA)GTACCCGAGAAAGCTTGTCACAGATGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613141019_i5ya TGTAATTTTTGTATATAATGGGCCAAATTAAG(+AGGACA)GATCCTGCTCTCTTTCTTTTGGAGGTAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613141018_9tjq ACCTCAAACTACCTCCAAAAGAAAGAGAGCAG(+AGGACA)GATCCTTAATTTGGCCCATTATATACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613141017_h8rr GCATGTATTCAGTTTTAGAATCACTTTTACTG(+AGGACA)GATCCTGTAGTAATGTCTAGAAGTATCATAT NGG 10 8 17 8 24 0 100 5 10
    240613141016_1w8b AATAGATATGATACTTCTAGACATTACTACAG(+AGGACA)GATCCAGTAAAAGTGATTCTAAAACTGAATA NGG 10 8 17 8 24 0 100 5 10
    240613141014_ah2g TTACTTGAAAATTGGACATAGTACTACCAGAG(+AGGACA)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613141012_h92p TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+AGGACA)GATCCTCTGGTAGTACTATGTCCAATTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613141009_8qxb GGATGGAGCATCTAGAGACTGTCATACCCGGG(+AGGACA)GATCCATCCCATTATTAGCTCCAAACGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613141008_vw3g TGGTTTCAGCGTTTGGAGCTAATAATGGGATG(+AGGACA)GATCCCCGGGTATGACAGTCTCTAGATGCTC NGG 10 8 17 8 24 0 100 5 10
    240613141006_esea ACTCATAACCTAGCTAAGAATTTTTGAGTCTG(+AGGACA)GTACCCATGGAGCACAAAAGCATTACAGAAA NGG 10 8 17 8 24 0 100 5 10