Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141141_yac0 TGGGCTAGACCACTTGTCCAGAGAACCCCTTG(+AGGACA)GATCCACCTATTCCCTCAGTGCTGGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240613141140_u9dl GGCAGGAAGATGGAGGATTCTTCCAAACATTG(+AGGACA)GATCCAGGTGACCCTTCTTCCTTGACCATCC NGG 10 8 17 8 24 0 100 5 10
    240613141138_lx5p GACAAGGATGGTCAAGGAAGAAGGGTCACCTG(+AGGACA)GATCCAATGTTTGGAAGAATCCTCCATCTTC NGG 10 8 17 8 24 0 100 5 10
    240613141137_eqt3 AGTAGATGCTCTTGGCTAATGGGTAAGGCGTG(+AGGACA)GTACCAGAAGCCTATGGCTTTTAGTTATGGT NGG 10 8 17 8 24 0 100 5 10
    240613141135_odmc CCACAACCATAACTAAAAGCCATAGGCTTCTG(+AGGACA)GTACCACGCCTTACCCATTAGCCAAGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613141134_9uym GGCGTCCTGGGATGGCACAGCCTCCCTCTGAG(+AGGACA)GTACCTAGGGAGATGTTCTAAGACCTGGGCG NGG 10 8 17 8 24 0 100 5 10
    240613141131_c2no CTGTGCGCCCAGGTCTTAGAACATCTCCCTAG(+AGGACA)GTACCTCAGAGGGAGGCTGTGCCATCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613141129_7lgd CCCAGCTCCAGTTGGCTACACGGCTACACGCG(+AGGACA)GATCCCTGCTCTGGCCCACTCACCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613141127_8z0s GTAGGCAGGCAGGGTGAGTGGGCCAGAGCAGG(+AGGACA)GATCCGCGTGTAGCCGTGTAGCCAACTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613141126_l3n3 GCCAGTCATCTGATGTGTCAAAAGTCTTCAAG(+AGGACA)GATCCTTTGCTAGAGCTTGTCTGCAGATGGA NGG 10 8 17 8 24 0 100 5 10