Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141350_uv3p GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AGGACA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613141348_breg CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AGGACA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613141346_l6d6 GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AGGACA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613141345_ec0w CATGTCTCTGCAGAGGCCAGAGGAGGGTATTG(+AGGACA)GATCCCTAGAAGCTGGAGTTAGAATAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240613141343_n48j GAGACTTACCTATTCTAACTCCAGCTTCTAGG(+AGGACA)GATCCAATACCCTCCTCTGGCCTCTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613141342_84e7 GTTCCAGCAAACAGGAAATGGGGATGCTGAAG(+AGGACA)GATCCCAGGGAGGGGACGTTACAACCACAGC NGG 10 8 17 8 24 0 100 5 10
    240613141339_uulg AATCAGCTGTGGTTGTAACGTCCCCTCCCTGG(+AGGACA)GATCCTTCAGCATCCCCATTTCCTGTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613141337_clqx TGTAACTCTCCCAGGCCCCAGAATAGCTCCTG(+AGGACA)GATCCAAGCCCGGGGCCCGGCCTGGGACACG NGG 10 8 17 8 24 0 100 5 10
    240613141336_4ur4 AGCGCCGTGTCCCAGGCCGGGCCCCGGGCTTG(+AGGACA)GATCCAGGAGCTATTCTGGGGCCTGGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613141333_qigu CAGTGGGAAGGAGGGGACCTCCCATGCCCAAG(+AGGACA)GATCCCCAGCGCCAGGGGACAGTGCCCAGGG NGG 10 8 17 8 24 0 100 5 10