Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141437_lh2e GCCACTTCTGGGGAGTCTACTAGACCTCTTTG(+AGGACA)GTACCCAAAACTCTCTGACAAGCCCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613141436_53nv CACCTGCAACCTTCCACGGTGCATGCTCTGTG(+AGGACA)GATCCCTCCTGGGCTTGTCAGAGAGTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613141434_292h GGTACCCAAAACTCTCTGACAAGCCCAGGAGG(+AGGACA)GATCCACAGAGCATGCACCGTGGAAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240613141432_ywdf CCCATTACGCCTGGTACAAGTCTGTCCTCAGG(+AGGACA)GATCCAGTGTGAGAAATACAGATGGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240613141431_fozw TGCGAGGCCATCCATCTGTATTTCTCACACTG(+AGGACA)GATCCCTGAGGACAGACTTGTACCAGGCGTA NGG 10 8 17 8 24 0 100 5 10
    240613141429_yb7x GGGGGGAACGGCAGCTGAGCAGGTGTAGGGTG(+AGGACA)GATCCTGCTGGTGACCAGGGCAGTCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240613141428_w5d6 GCTTCATGGATGACTGCCCTGGTCACCAGCAG(+AGGACA)GATCCACCCTACACCTGCTCAGCTGCCGTTC NGG 10 8 17 8 24 0 100 5 10
    240613141427_ujvb CAGTCCAGTCAAGTGAGAAATGGAAAGAGTTG(+AGGACA)GTACCACGGGGCCAGGGAAACAAAGTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613141424_fham AGTCCATCTCACTTTGTTTCCCTGGCCCCGTG(+AGGACA)GTACCAACTCTTTCCATTTCTCACTTGACTG NGG 10 8 17 8 24 0 100 5 10
    240613141422_2ppf ATGTGCCAGGCAAGCACAGTGCTCGGAGGATG(+AGGACA)GATCCATTGTCTACCTGCCTGGTCATCTTCC NGG 10 8 17 8 24 0 100 5 10