Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141616_n94f CTTGTGCCCTCAGAAGTCAGAAGACGGCATGG(+AGGACA)GATCCCCTGGACCTGGAGTTAGGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613141614_itq4 GCACCAGCAGCATCCAATCAACCTGTACTATG(+AGGACA)GATCCTCCAACATCTAAGCAATAATGCCCTA NGG 10 8 17 8 24 0 100 5 10
    240613141613_cpn3 AGCTGTAGGGCATTATTGCTTAGATGTTGGAG(+AGGACA)GATCCATAGTACAGGTTGATTGGATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613141612_phbd ACACATTTGTGATAAGACAAGGAAGTCTACAG(+AGGACA)GATCCTCCATCTCTTAGTCTTGCTGAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613141610_4o8c TGAAATGACCTCAGCAAGACTAAGAGATGGAG(+AGGACA)GATCCTGTAGACTTCCTTGTCTTATCACAAA NGG 10 8 17 8 24 0 100 5 10
    240613141609_txsq GGAGGGGACCCGATTCCCGCCAAATAATCTCG(+AGGACA)GATCCAGTAGCAGGGTGAGCACGTGATGGCT NGG 10 8 17 8 24 0 100 5 10
    240613141607_1aw7 TCTGAAGCCATCACGTGCTCACCCTGCTACTG(+AGGACA)GATCCGAGATTATTTGGCGGGAATCGGGTCC NGG 10 8 17 8 24 0 100 5 10
    240613141606_fjho AAAGCCTTGTGACATAGCTGTCCAAACATTTG(+AGGACA)GATCCTTTAGCCACAGGATGACCCACTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613141605_8o3n TCTTAGACAAGTGGGTCATCCTGTGGCTAAAG(+AGGACA)GATCCAAATGTTTGGACAGCTATGTCACAAG NGG 10 8 17 8 24 0 100 5 10
    240613141603_ie79 TATTTCTCTCATTTGAAGATTTTCTCAACAGG(+AGGACA)GTACCTCCTCTGAACAACAAGCTTCCCACAT NGG 10 8 17 8 24 0 100 5 10