Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140213_y2wp GGTGTGAATGTCCTAGAATTCTTGTGGGGCAG(+AGGACA)GTACCACTGGGTTCCATTCTGCCAGGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613140212_8ee3 GTTGGCCTTCTCACTTTGATAGTTTAACTCAG(+AGGACA)GTACCCAGTCTTCACTCCATCCCCAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613140210_hbbv GAACAAGCACCTGGGGATGGAGTGAAGACTGG(+AGGACA)GTACCTGAGTTAAACTATCAAAGTGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613140209_9gkh AACTTCAAAGCTTTATTCTGTCTGCCTGAGAG(+AGGACA)GATCCATGGAAGATGATTCTCCCAAAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613140207_sdi8 TTTTCTGCTTTTTGGGAGAATCATCTTCCATG(+AGGACA)GATCCTCTCAGGCAGACAGAATAAAGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613140206_445e GAAGGCTTTCTACTCCTTAACAGGTACAGTTG(+AGGACA)GATCCATCGCTGATGGTGTTTCTTTAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613140205_jdyj GGGATGGGGCTAAAGAAACACCATCAGCGATG(+AGGACA)GATCCAACTGTACCTGTTAAGGAGTAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613140204_1jv1 GGAGGGATGCAGACTGAGGCTGCTCAATTCTG(+AGGACA)GTACCCCATAGTTCCTGTTGGCCTTAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613140202_syqw GTTTTGGCTTTAAGGCCAACAGGAACTATGGG(+AGGACA)GTACCAGAATTGAGCAGCCTCAGTCTGCATC NGG 10 8 17 8 24 0 100 5 10
    240613140201_bg3y AACAGCATATCCAACAAGGGAGCTAAGAACTG(+AGGACA)GATCCATGAGCATCCTAAGGATGAGGCCTGG NGG 10 8 17 8 24 0 100 5 10