Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140128_er5s ACAGGGTAACAGGGCCTGGGGTGGGGACAAGG(+AGGACA)GTACCCTGTTCAGAGGTGACCTGCAAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240613140126_vjbu TCTCCTGTGTAGACTAGCTGGAGGTCCCTGGG(+AGGACA)GATCCCCAGGGAAAGGACGCTCTGCCGCGCA NGG 10 8 17 8 24 0 100 5 10
    240613140123_dhz4 GAGATTGCGCGGCAGAGCGTCCTTTCCCTGGG(+AGGACA)GATCCCCAGGGACCTCCAGCTAGTCTACACA NGG 10 8 17 8 24 0 100 5 10
    240613140121_g7n3 CTTTGTCCTCGTCGAGCTGCTCTGAGCTGTGG(+AGGACA)GATCCTGTGAACAACTGTCGTCTTTCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613140120_4t7w TGAAGAGAAGGAAAGACGACAGTTGTTCACAG(+AGGACA)GATCCCACAGCTCAGAGCAGCTCGACGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613140119_sj33 CCAAAATGCCCGTCCTATAGAGTGTCTGATTG(+AGGACA)GATCCTCTGTTGCGTATTCTGATGGTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613140118_kvxh ACCAGCAGGCACCATCAGAATACGCAACAGAG(+AGGACA)GATCCAATCAGACACTCTATAGGACGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613140116_1ev7 GCTGTTGTCAGTCTCCATGAAAGCTCTAGCTG(+AGGACA)GTACCTTGGGTCTTGTCCTGACACCCGTATC NGG 10 8 17 8 24 0 100 5 10
    240613140114_9nni TCACAGATACGGGTGTCAGGACAAGACCCAAG(+AGGACA)GTACCAGCTAGAGCTTTCATGGAGACTGACA NGG 10 8 17 8 24 0 100 5 10
    240613140113_hc5x GTGTCTCATAAAGCAGCTGTTCAGGATGTGTG(+AGGACA)GATCCTCTGCCATTGCTCTCCCGCGTGGGGA NGG 10 8 17 8 24 0 100 5 10