Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140112_xllr GAGCTTCCCCACGCGGGAGAGCAATGGCAGAG(+AGGACA)GATCCACACATCCTGAACAGCTGCTTTATGA NGG 10 8 17 8 24 0 100 5 10
    240613140111_es2z CCCTATGTTTCCACTCGCAATCATATACTTAG(+AGGACA)GTACCTTTGACCCCATGGAAAAAAATATCTA NGG 10 8 17 8 24 0 100 5 10
    240613140109_hmj7 AATGTTAGATATTTTTTTCCATGGGGTCAAAG(+AGGACA)GTACCTAAGTATATGATTGCGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240613140108_o771 ACCCTCAGCCTTGTCAACAGCTGAAAAATCTG(+AGGACA)GTACCCAAAAGTCAAGAGGGTTTAAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240613140107_75ir TTTATTAAGTCTTAAACCCTCTTGACTTTTGG(+AGGACA)GTACCAGATTTTTCAGCTGTTGACAAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613140105_5evk AAGAAAAGGGATGTCCAGTGCAGAGTCTACAG(+AGGACA)GATCCAAAGTGAAGAACTGCAGTGGACACTC NGG 10 8 17 8 24 0 100 5 10
    240613140104_9p30 TGAATGAGTGTCCACTGCAGTTCTTCACTTTG(+AGGACA)GATCCTGTAGACTCTGCACTGGACATCCCTT NGG 10 8 17 8 24 0 100 5 10
    240613140103_45i1 ATGGTATGAATTGTATTGTAATGCTAAATCTG(+AGGACA)GTACCTGAAGGTCTAGGCCTGTGAGTGAATT NGG 10 8 17 8 24 0 100 5 10
    240613140102_3iwf GTGAGAATTCACTCACAGGCCTAGACCTTCAG(+AGGACA)GTACCAGATTTAGCATTACAATACAATTCAT NGG 10 8 17 8 24 0 100 5 10
    240613140100_u4en TTTGTAAGCCAGTTGGGGATAAGCTTATATTG(+AGGACA)GTACCACATGGGAGAAAAAGAACCAGGACCT NGG 10 8 17 8 24 0 100 5 10