Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135322_kvco ATACTTTAGTCAAGTATACCCTGACTTTCATG(+AGGACA)GTACCAATAAAGTCAGAACTCCATCATAATT NGG 10 8 17 8 24 0 100 5 10
    240613135320_f1no CAGCCTTCCAAGTCATGAACCTTCTTTGAGAG(+AGGACA)GATCCACCCCACTAGTCCTTCCCCCTCGCAC NGG 10 8 17 8 24 0 100 5 10
    240613135318_s4vv GGTGGGTGCGAGGGGGAAGGACTAGTGGGGTG(+AGGACA)GATCCTCTCAAAGAAGGTTCATGACTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240613135317_yvh7 CTTTACCTGTTCCTCTAGCAGCATCAGTGTAG(+AGGACA)GTACCCCACCCGGACCACTGACACCCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613135315_rcux CTTGGCAACAGGGTGTCAGTGGTCCGGGTGGG(+AGGACA)GTACCTACACTGATGCTGCTAGAGGAACAGG NGG 10 8 17 8 24 0 100 5 10
    240613135314_m8h8 AGAACTGCTGCAGCAAACACTGGTTCTAGCAG(+AGGACA)GATCCTCAGGAGTTGCAATGCATTAAGATAG NGG 10 8 17 8 24 0 100 5 10
    240613135312_1e6a CTGTGCTATCTTAATGCATTGCAACTCCTGAG(+AGGACA)GATCCTGCTAGAACCAGTGTTTGCTGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613135310_20ax CTCACCAAGCTCTAGGCATGGTTCCCTGAGGG(+AGGACA)GTACCCAGCCCTGGATATCTCCAGGGATCAC NGG 10 8 17 8 24 0 100 5 10
    240613135308_gps5 AAGTAGTGATCCCTGGAGATATCCAGGGCTGG(+AGGACA)GTACCCCTCAGGGAACCATGCCTAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613135307_31mz TGAAATATCTGATATGTGAGCCTCGCAGGGTG(+AGGACA)GATCCTTCAAGACCCATAGGTTGAGAATCAC NGG 10 8 17 8 24 0 100 5 10