Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135404_0xw1 ATGGACCAGCTAACCCTTATTCAGACCTTGGG(+AGGACA)GATCCATGCTCCAGTGTCTCCTCACTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613135402_1ye8 GGAGATTCATCAGAACTCAGCACACGCTGGTG(+AGGACA)GATCCACAGCACTGTCATGTCTGTTATGAAT NGG 10 8 17 8 24 0 100 5 10
    240613135401_v7yz GTGTCATTCATAACAGACATGACAGTGCTGTG(+AGGACA)GATCCACCAGCGTGTGCTGAGTTCTGATGAA NGG 10 8 17 8 24 0 100 5 10
    240613135359_i15j CCTAACCCATGCGCTGCTGGTGGGTACTGAAG(+AGGACA)GTACCCACTTGGACTTGGAGGGGTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613135357_728n GTCATGGAGCCTACCCCTCCAAGTCCAAGTGG(+AGGACA)GTACCTTCAGTACCCACCAGCAGCGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240613135356_2zyc TTACTTTGCGTGGCTGGGAGAAGAGTAGACTG(+AGGACA)GATCCTGACCTTTCTGCACACTTCCAGAACC NGG 10 8 17 8 24 0 100 5 10
    240613135355_jkly GCGTTGGTTCTGGAAGTGTGCAGAAAGGTCAG(+AGGACA)GATCCAGTCTACTCTTCTCCCAGCCACGCAA NGG 10 8 17 8 24 0 100 5 10
    240613135354_csf9 GTTCTGCCAAGACCGGCAGGGTAAACAAACAG(+AGGACA)GTACCACTCTCTCCCGGGAACCCTTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613135352_zvo2 ACAACAGAAGAAAGGGTTCCCGGGAGAGAGTG(+AGGACA)GTACCTGTTTGTTTACCCTGCCGGTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613135351_4izh CAGGAAAGGTTCCGTTTATAAACAAGAAACTG(+AGGACA)GTACCAGAAAATTGCAGAGAAGCACCAACGT NGG 10 8 17 8 24 0 100 5 10