Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135512_188p AGAAGGAGGCTGGGATGGGCAGGGCTAGAAAG(+AGGACA)GTACCCAGGAAATGACTTCTTGCCCAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613135511_twv9 TTCTCCCCACAGCCACAGGGAAGTTGACACTG(+AGGACA)GATCCCTCTGCTTGTCCCCATCTTTTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613135509_hyyb AGGTGAGCAAAAAAGATGGGGACAAGCAGAGG(+AGGACA)GATCCAGTGTCAACTTCCCTGTGGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613135507_u9qn AGCCAACAGACTTGAAAAGGCCCCTTGTCTAG(+AGGACA)GTACCCCACGAAAATAAACTTTGAAAACGTA NGG 10 8 17 8 24 0 100 5 10
    240613135505_mb9x CTGGATACGTTTTCAAAGTTTATTTTCGTGGG(+AGGACA)GTACCTAGACAAGGGGCCTTTTCAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613135504_i0xn GAGAACAGAAGCGCCTGGGGAAGAAGGGTGAG(+AGGACA)GATCCCCCAAATCAGAGTATGCCTGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240613135502_yd1h TCAATCACTCCCAGGCATACTCTGATTTGGGG(+AGGACA)GATCCTCACCCTTCTTCCCCAGGCGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613135459_ozl5 GGGATGGGCTTAGACCATTAAAGGGACCAGGG(+AGGACA)GATCCTGGCATGTGCAAGTGGTGATGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240613135457_hnw7 AACCCACGGCCATCACCACTTGCACATGCCAG(+AGGACA)GATCCCCTGGTCCCTTTAATGGTCTAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613135456_exbj GGAAAATGTCTCCGTGGATCACTCAGTGATAG(+AGGACA)GATCCGCCTCTTGAGTGCTGTCAGTGGCTAG NGG 10 8 17 8 24 0 100 5 10