Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135438_vy8w TTCTCTGCCTCCCAAGTACTGGGATTAGAAGG(+AGGACA)GTACCCCACCACACCCAGCTGGGCTTCATTT NGG 10 8 17 8 24 0 100 5 10
    240613135436_8t6y GCTGCTTCAACCAGACTGAGCTTGGTGCCTGG(+AGGACA)GTACCCAGTGATGAGCCAAGTAAGGTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613135434_eei2 GCACTTGCCTACCTTACTTGGCTCATCACTGG(+AGGACA)GTACCCAGGCACCAAGCTCAGTCTGGTTGAA NGG 10 8 17 8 24 0 100 5 10
    240613135433_47pe CCTAACCCATGCGCTGCTGGTGGGTACTGAAG(+AGGACA)GTACCCACTTGGACTTGGAGGGGTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613135431_o8n2 GTCATGGAGCCTACCCCTCCAAGTCCAAGTGG(+AGGACA)GTACCTTCAGTACCCACCAGCAGCGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240613135428_464d CAAAGTGCTCAGAGTTCCAGCCCTGGGCCCCG(+AGGACA)GTACCCATATCCATACCACTCCCTGGCTCGG NGG 10 8 17 8 24 0 100 5 10
    240613135427_iirl GTAAACCGAGCCAGGGAGTGGTATGGATATGG(+AGGACA)GTACCGGGGCCCAGGGCTGGAACTCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613135425_29ka GGAGACAAGATATGACAGGAAGAAACTTTTAG(+AGGACA)GTACCTGGGCAGAAAGAGCTGTTGTTTGAAA NGG 10 8 17 8 24 0 100 5 10
    240613135423_z2xn GGCCCTTTCAAACAACAGCTCTTTCTGCCCAG(+AGGACA)GTACCTAAAAGTTTCTTCCTGTCATATCTTG NGG 10 8 17 8 24 0 100 5 10
    240613135422_w9cm GAATGGCAAGAGGATCAAGGTCAGAATGTCTG(+AGGACA)GTACCGTCTGTGGAATCTAAGATAGGGCTCC NGG 10 8 17 8 24 0 100 5 10