Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135531_yl2t CTCTTTGCATTCTGCATGAAAGTTTCTGCATG(+AGGACA)GTACCAAGTAGACAGTACCTGTGAGTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240613135528_8n5l TTTAATCTTTTCCCTCTGTCAGAGTACCCAGG(+AGGACA)GATCCATGAAGTGCCTGTCGCTCGAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240613135526_s42l GCCTGGAGTCTTCGAGCGACAGGCACTTCATG(+AGGACA)GATCCCTGGGTACTCTGACAGAGGGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613135525_xp43 CCCACTTCTCAGGAATGACCTTCCACAGCTGG(+AGGACA)GTACCCCTTAAATCTGCTATGTTCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613135523_hxjj ACCTGGAGGCAGGAACATAGCAGATTTAAGGG(+AGGACA)GTACCCAGCTGTGGAAGGTCATTCCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613135521_3kr7 GTGTAACCACGGCCACCTCATTGCAGAGCAGG(+AGGACA)GATCCAGGTTAGGGAAGTATAGAGTCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613135519_3l6x TCTGTTCTGGGACTCTATACTTCCCTAACCTG(+AGGACA)GATCCCTGCTCTGCAATGAGGTGGCCGTGGT NGG 10 8 17 8 24 0 100 5 10
    240613135517_vlpg GGCACAGTGGTAGAGCCACTCTGGATTGATGG(+AGGACA)GATCCAATGGAATGACCTAGAGCTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240613135515_47yy GGGGTTGCATTTAGCTCTAGGTCATTCCATTG(+AGGACA)GATCCCATCAATCCAGAGTGGCTCTACCACT NGG 10 8 17 8 24 0 100 5 10
    240613135513_n5px TTTGCTCACCTGGGCAAGAAGTCATTTCCTGG(+AGGACA)GTACCTTTCTAGCCCTGCCCATCCCAGCCTC NGG 10 8 17 8 24 0 100 5 10