Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135559_v000 GTGTGCATTCCATCAATATAGTTTAAAATAGG(+AGGACA)GATCCAGGTTCGGCAGCATGGAAACGAGATT NGG 10 8 17 8 24 0 100 5 10
    240613135558_tqkt TTAAGCTGCAAATCTTCTCAGGTGTTCATATG(+AGGACA)GTACCTATCCATAGATACTCACATAGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613135556_5pn5 TAGGGTTTTACTATGTGAGTATCTATGGATAG(+AGGACA)GTACCATATGAACACCTGAGAAGATTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613135555_wjj0 AAGATAAGGATAAGGAAGTTGTAGTTTATATG(+AGGACA)GATCCCAAAGTCTTGTGGTTTTGCTGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613135553_uujx AAACTGGGAACAGCAAAACCACAAGACTTTGG(+AGGACA)GATCCATATAAACTACAACTTCCTTATCCTT NGG 10 8 17 8 24 0 100 5 10
    240613135552_u8d7 CCAGCCCTTTGGAGTAGCTCAAATCATGTGTG(+AGGACA)GATCCCAAACATTGAAACAAGAAGCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240613135550_jk3o TTAATGCTCCAGCTTCTTGTTTCAATGTTTGG(+AGGACA)GATCCACACATGATTTGAGCTACTCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613135549_6gaw AGCACCCTTGTGTCTCTGGCTTCCTGTCACTG(+AGGACA)GATCCAGTACCCCTCCATGCTTGGGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613135548_a9do TAAAACAAGGACCCAAGCATGGAGGGGTACTG(+AGGACA)GATCCAGTGACAGGAAGCCAGAGACACAAGG NGG 10 8 17 8 24 0 100 5 10
    240613135546_44f9 CTTAATATTTTCAAACTAATAGTTTATTATAG(+AGGACA)GTACCATAAATCTTTAAATAAACACTATTTT NGG 10 8 17 8 24 0 100 5 10