Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135545_1hmm AAAATAAAATAGTGTTTATTTAAAGATTTATG(+AGGACA)GTACCTATAATAAACTATTAGTTTGAAAATA NGG 10 8 17 8 24 0 100 5 10
    240613135544_r4ib TGGAATGTCTTGGAGGCCAGAGGAGGGTGTTG(+AGGACA)GATCCCTTAGGACTGGAGTTAAAGACGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613135542_yr7o CTCACCTCTCGTCTTTAACTCCAGTCCTAAGG(+AGGACA)GATCCAACACCCTCCTCTGGCCTCCAAGACA NGG 10 8 17 8 24 0 100 5 10
    240613135541_d5ri ATCTAGGCTCTGGTCCTCATCTGGGGTCAAAG(+AGGACA)GATCCCTTAGGAGGGGTCAGTTTTGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613135539_15al ACAATCAGAAGCAAAACTGACCCCTCCTAAGG(+AGGACA)GATCCTTTGACCCCAGATGAGGACCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613135538_8ezh TTTAAAGAACCACAGTGTGCAAATGTTTCAGG(+AGGACA)GATCCTAAGCAATGCCCCAACACATGAACAG NGG 10 8 17 8 24 0 100 5 10
    240613135536_pwva CCAGCCTGTTCATGTGTTGGGGCATTGCTTAG(+AGGACA)GATCCCTGAAACATTTGCACACTGTGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613135535_87z1 CCTGTAAGTACTCCCTGAGAATGCCCATTCAG(+AGGACA)GATCCCCTGAAAGGCAAATGACAGCTATACA NGG 10 8 17 8 24 0 100 5 10
    240613135533_9my7 GGTCATGTATAGCTGTCATTTGCCTTTCAGGG(+AGGACA)GATCCTGAATGGGCATTCTCAGGGAGTACTT NGG 10 8 17 8 24 0 100 5 10
    240613135532_d3rh GATTAGTTTAACTCACAGGTACTGTCTACTTG(+AGGACA)GTACCATGCAGAAACTTTCATGCAGAATGCA NGG 10 8 17 8 24 0 100 5 10