Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135307_31mz TGAAATATCTGATATGTGAGCCTCGCAGGGTG(+AGGACA)GATCCTTCAAGACCCATAGGTTGAGAATCAC NGG 10 8 17 8 24 0 100 5 10
    240613135306_fb2n GAACAGTGATTCTCAACCTATGGGTCTTGAAG(+AGGACA)GATCCACCCTGCGAGGCTCACATATCAGATA NGG 10 8 17 8 24 0 100 5 10
    240613135304_e0kk GCCTGACTTTTTCCTGTTTTGAGCCATCTGTG(+AGGACA)GTACCTGTCCAAGGTGGTAACTCCACTATAG NGG 10 8 17 8 24 0 100 5 10
    240613135303_uxyt CACTACTATAGTGGAGTTACCACCTTGGACAG(+AGGACA)GTACCACAGATGGCTCAAAACAGGAAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613135301_37ls TGGACTGTCCACTAGAGTTGGACAGTACTGGG(+AGGACA)GTACCCAGGTCCACCCCTTGGAATACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613135259_7tgx TCCAGACAGGGTATTCCAAGGGGTGGACCTGG(+AGGACA)GTACCCCAGTACTGTCCAACTCTAGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240613135257_ojml TAGCAAGCTCACTCACCAAGCCGCACTGGGTG(+AGGACA)GATCCATCTTTGGTTTGTTGGAGAGAACAGA NGG 10 8 17 8 24 0 100 5 10
    240613135256_d8nl TAGAGTCTGTTCTCTCCAACAAACCAAAGATG(+AGGACA)GATCCACCCAGTGCGGCTTGGTGAGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613135254_yk0k TTGTTTCCTCCCAAATACTCCCTGAACGGGAG(+AGGACA)GATCCCCTTCTGCTCCATCAGGACTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240613135252_gda3 GGCTTCAGTGGAGTCCTGATGGAGCAGAAGGG(+AGGACA)GATCCTCCCGTTCAGGGAGTATTTGGGAGGA NGG 10 8 17 8 24 0 100 5 10