Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134938_kxr6 CCTGTGGTCACCGGCGACAATATTCTAGAGAG(+AGGACA)GATCCCGTTTCTAAGGATGGTGAGTTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613134937_z078 TTACTTTGATGGCTTCTCCAGGTTTTTGTCAG(+AGGACA)GTACCACATGTAGAATTACATTTTTCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613134936_gj61 AATCTTTTAAGAAAAATGTAATTCTACATGTG(+AGGACA)GTACCTGACAAAAACCTGGAGAAGCCATCAA NGG 10 8 17 8 24 0 100 5 10
    240613134935_k59v CCTCCATCCGGAGGAAGCAACAGCTGAATCAG(+AGGACA)GATCCGCAGCGTAAGGGGTCAGCTAATCAGT NGG 10 8 17 8 24 0 100 5 10
    240613134933_l8an CTCCCACTGATTAGCTGACCCCTTACGCTGCG(+AGGACA)GATCCTGATTCAGCTGTTGCTTCCTCCGGAT NGG 10 8 17 8 24 0 100 5 10
    240613134932_he5q AGCTAGACATTTATTGATAATACATTACAGTG(+AGGACA)GATCCAAAATCTAGCATTATTTTAGTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613134931_703g CCTGTTCACCACTAAAATAATGCTAGATTTTG(+AGGACA)GATCCACTGTAATGTATTATCAATAAATGTC NGG 10 8 17 8 24 0 100 5 10
    240613134929_5owz ATGTGCCCATCAGGGGGCCGTCTTAACCTTTG(+AGGACA)GTACCATCTTCTTCAAAGCTGCATTAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613134928_d06k TGCTCTCTTTTAATGCAGCTTTGAAGAAGATG(+AGGACA)GTACCAAAGGTTAAGACGGCCCCCTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240613134925_h2dx TGGGCTAGGATCACTTGCCACAGAGCCCCAGG(+AGGACA)GATCCACCTGTCTCTGTGTCCCCAGCACTGG NGG 10 8 17 8 24 0 100 5 10