Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134857_t1xe GGCAGAATGAGATAATGGTGAGTGAAAATCAG(+AGGACA)GTACCATTCCAAATAAAACCAGAGTACCATC NGG 10 8 17 8 24 0 100 5 10
    240613134855_tub7 CATGGCAGGTGGCTCTAAGGACACCTAGCATG(+AGGACA)GTACCCATTTGATTAAGAGAAGAAGATGCCT NGG 10 8 17 8 24 0 100 5 10
    240613134854_uj0d CAGGAAGGCATCTTCTTCTCTTAATCAAATGG(+AGGACA)GTACCATGCTAGGTGTCCTTAGAGCCACCTG NGG 10 8 17 8 24 0 100 5 10
    240613134852_2k92 TTAGGCTGTTTGGCAGATACAACAGAGTCCTG(+AGGACA)GATCCTCCTTGCCTCCTTCAGAAGCTCCGTA NGG 10 8 17 8 24 0 100 5 10
    240613134850_cwai ACGCCTACGGAGCTTCTGAAGGAGGCAAGGAG(+AGGACA)GATCCAGGACTCTGTTGTATCTGCCAAACAG NGG 10 8 17 8 24 0 100 5 10
    240613134849_5ru1 ACTGTAAGAATAACAGACACCCCTTTTTGCTG(+AGGACA)GTACCACCCTCCTCGAGTCCCCATGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613134847_hrr4 AGGGTGCCAACCATGGGGACTCGAGGAGGGTG(+AGGACA)GTACCAGCAAAAAGGGGTGTCTGTTATTCTT NGG 10 8 17 8 24 0 100 5 10
    240613134846_axrg TGTCTACACGTCACTACTTACAATTTTAACTG(+AGGACA)GTACCAAGTCTGTGCCCAGAGGCAACAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613134845_9fcj GGATTAGCTTGTTGCCTCTGGGCACAGACTTG(+AGGACA)GTACCAGTTAAAATTGTAAGTAGTGACGTGT NGG 10 8 17 8 24 0 100 5 10
    240613134843_hayf CTTCATACAGCAGTCATCTGCTCGTATGTTTG(+AGGACA)GATCCCCTTTTAAACATTCAACCCTGGAGGT NGG 10 8 17 8 24 0 100 5 10