Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134756_69if CTCCTTCCCCTCCCCCTCCCCCCTCCCTCCAG(+AGGACA)GATCCTGCTGGTATTTCTAGATATGTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240613134754_2vig TAAACAACTAATGTGGAAGGCTGATGCAGGAG(+AGGACA)GATCCATAGGAAACAGAAACAGCCCAAATGC NGG 10 8 17 8 24 0 100 5 10
    240613134753_s0ob GGGGGGCATTTGGGCTGTTTCTGTTTCCTATG(+AGGACA)GATCCTCCTGCATCAGCCTTCCACATTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613134751_k5ir CCACCTGGATCTCAGAACTTAATTCAAGTGGG(+AGGACA)GTACCATATGTGATGGGTGTCTGTGATGGAG NGG 10 8 17 8 24 0 100 5 10
    240613134750_ia5x CGTTTCTCCATCACAGACACCCATCACATATG(+AGGACA)GTACCCCACTTGAATTAAGTTCTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240613134747_gs3b TAGGAGAAGTCATAATTAAATCCTGCAGTGGG(+AGGACA)GTACCAGTGACCCCAGTCACGTTGGACTAAG NGG 10 8 17 8 24 0 100 5 10
    240613134746_c1b4 AGCTTCTTAGTCCAACGTGACTGGGGTCACTG(+AGGACA)GTACCCCACTGCAGGATTTAATTATGACTTC NGG 10 8 17 8 24 0 100 5 10
    240613134745_c7w6 CACTTAATTCTCTGACCGGGCCAGCATCATGG(+AGGACA)GTACCTGCTCCCCTGTGTACCCCATCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613134744_6osn AAACCTTAAGGATGGGGTACACAGGGGAGCAG(+AGGACA)GTACCCATGATGCTGGCCCGGTCAGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240613134741_812r ATGGTGAAGGGAAGTCGGGAGGGGGGGAAGGG(+AGGACA)GATCCGAACACAGTTGACTTTTATTTTGTAA NGG 10 8 17 8 24 0 100 5 10