Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134649_99jd TTCTTAGACCCACCCGCGGGAACCGTTCACAG(+AGGACA)GTACCCTCCCTCGGGCCCTTTTGATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613134648_2l5v CAGGCTGGCTGCGAAAGGAGGCTACACATATG(+AGGACA)GATCCACCTGACTGTAATCAGTGAATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240613134646_lyz5 AGAAGTTGAGATTCACTGATTACAGTCAGGTG(+AGGACA)GATCCATATGTGTAGCCTCCTTTCGCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613134645_n3qj GCTGGGGAGGCCAGTGTATCTGCTGGAGTCTG(+AGGACA)GATCCTGACATTTACCTATTTCTTATTGGCA NGG 10 8 17 8 24 0 100 5 10
    240613134643_gwod AGGATTGCCAATAAGAAATAGGTAAATGTCAG(+AGGACA)GATCCAGACTCCAGCAGATACACTGGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613134641_jebz AGTCCCAACACCTATGCGTGTTCCCAACTCCG(+AGGACA)GATCCTGTTAATACTATGGTTAAAAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613134640_bvwx TATGGAACCACTTTTAACCATAGTATTAACAG(+AGGACA)GATCCGGAGTTGGGAACACGCATAGGTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613134638_7xlj TCTGGGCAGTAAGGACCCTATCACTGAGCCAG(+AGGACA)GTACCTTGTCCTCTTCTTATTTTGAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240613134637_rqzj AAAATGATGCTTCAAAATAAGAAGAGGACAAG(+AGGACA)GTACCTGGCTCAGTGATAGGGTCCTTACTGC NGG 10 8 17 8 24 0 100 5 10
    240613134635_mz1h ACAGCCAGCACAGGAAGGAGCCAGTCATTTCG(+AGGACA)GTACCTCTGAGGTTCTGAGAGTCGAGGGATA NGG 10 8 17 8 24 0 100 5 10