Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134706_gye0 ATATTTTATTTTTCATACATGTTTGAATTGCG(+AGGACA)GATCCATGGATGTTCCATCTGTAGGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240613134704_57cp GGACAGACAGGAAGTACACATGAGACACCAAG(+AGGACA)GATCCAAAACAACAGGCAGGGCTGGGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613134702_33hb GCTAATGCAGCCCAGCCCTGCCTGTTGTTTTG(+AGGACA)GATCCTTGGTGTCTCATGTGTACTTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613134701_mjuy AAGAGATTTGAAACACATTTGAGCTGTAAGTG(+AGGACA)GTACCTGAGGACCAGAAGATGGCCATGGGCG NGG 10 8 17 8 24 0 100 5 10
    240613134659_41va GAGGCCGCCCATGGCCATCTTCTGGTCCTCAG(+AGGACA)GTACCACTTACAGCTCAAATGTGTTTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613134658_b25a GGCCTAGTGTATCTATTATCTAGTTCTTTCGG(+AGGACA)GTACCAGGGTTCTCCCCCTATCCTGTGTTCA NGG 10 8 17 8 24 0 100 5 10
    240613134655_n84a TGCTCTGAACACAGGATAGGGGGAGAACCCTG(+AGGACA)GTACCCGAAAGAACTAGATAATAGATACACT NGG 10 8 17 8 24 0 100 5 10
    240613134653_kpgf CTCCAGCTTCCACCTCAGAGAAAACACCAAAG(+AGGACA)GTACCAAGAGCTAAGCCCAGGGGCCAACCAA NGG 10 8 17 8 24 0 100 5 10
    240613134652_7puy TGTTCTTGGTTGGCCCCTGGGCTTAGCTCTTG(+AGGACA)GTACCTTTGGTGTTTTCTCTGAGGTGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613134650_yp11 TAACTCCCAGAATCAAAAGGGCCCGAGGGAGG(+AGGACA)GTACCTGTGAACGGTTCCCGCGGGTGGGTCT NGG 10 8 17 8 24 0 100 5 10