Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134812_jk4u CCACAAGATGGCTCACAACCATCCATTAATGG(+AGGACA)GATCCAATGCCCTCTTCTGATGTGCCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240613134810_raku ATCACTCGCGAATTGCGTAAGCGGCCCTTCGG(+AGGACA)GTACCCGGGCAGAGAGAACTGCGCGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613134808_8gmv GGCAGCAAGCCCGCGCAGTTCTCTCTGCCCGG(+AGGACA)GTACCCGAAGGGCCGCTTACGCAATTCGCGA NGG 10 8 17 8 24 0 100 5 10
    240613134807_z432 AGAGGGATAGATGACTTGCGAAGGCCACACTG(+AGGACA)GTACCCTTTTCTGAGACACCCTTCCGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240613134805_74de AAAGAACCTACGGAAGGGTGTCTCAGAAAAGG(+AGGACA)GTACCAGTGTGGCCTTCGCAAGTCATCTATC NGG 10 8 17 8 24 0 100 5 10
    240613134804_bwq7 GGAGACACTGTACGGTTATTATGTCCTTACTG(+AGGACA)GATCCTGAGGGTCATTTTATCTATATGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613134802_tks6 TGTTGAAAGCATATAGATAAAATGACCCTCAG(+AGGACA)GATCCAGTAAGGACATAATAACCGTACAGTG NGG 10 8 17 8 24 0 100 5 10
    240613134800_1avj GTTACAATGGAGAAAACAGAAAAGGACTTTAG(+AGGACA)GTACCATTTATTTCCTCTGACAGTCAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613134759_38u7 AAGAGCTTTTTGACTGTCAGAGGAAATAAATG(+AGGACA)GTACCTAAAGTCCTTTTCTGTTTTCTCCATT NGG 10 8 17 8 24 0 100 5 10
    240613134758_9jxt ATAGTTAACCACATATCTAGAAATACCAGCAG(+AGGACA)GATCCTGGAGGGAGGGGGGAGGGGGAGGGGA NGG 10 8 17 8 24 0 100 5 10